TotalOmics
전장 전사체 생명정보 분석
1. Data filtering: Data filtering includes removing adaptors, contamination and low-quality reads from raw reads.
2. Standard bioinformatics analysis with reference (reference genes, genome sequences and gene annotation should be provided)
1. Assessment of sequencing (Alignment Statistics, Randomness assessment of sequencing, Distribution of reads on the reference genome)
2. Gene expression and annotation (Gene coverage and coverage depth)
3. Gene expression difference analysis
(two or more samples should be provided; if the alinment has been conducted more than 10 times, it will be charged as the custom bioinformatics analysis)
4. Refinement of gene structures (eukaryote ONLY)
5. Identification of alternative spliced transcripts (eukaryote ONLY)
6. Predication of novel transcripts
7. SNP analysis (eukaryote ONLY)
3. Standard bioinformatics analysis (de novo transcriptome assembly)
1. Statistics analysis and evaluation of data
2. Assembly results (Contig length distribution, Unigene length distribution)
3. Unigene function annotation
4. Unigene GO classification
5. Unigene COG analysis
6. Unigene metabolic pathway analysis
7. Protein coding region prediction (CDS)
8. Unigene expression difference analysis
(two or more samples should be provided; if the alignment has been conducted more than 10 times, it will be charged as the custom bioinformatics analysis)
9. Gene ontology (GO) classification of differentially expressed unigene (two or more samples should be provided) and Pathway enrichment analysis.
